The DMEK ‘S’ marker is designed to be used on the donor graft, through a 3mm diameter hole in the cornea created with a trephine. The ‘S’ is lightly marked on to the stromal side of descemet membrane (Not the endothelial side). Once the graft is inside the anterior chamber, the ‘S’ verifies that it is the right side up, before the graft is finally positioned.